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spin column yeast total rna purification kit  (Sangon Biotech)

 
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    Sangon Biotech spin column yeast total rna purification kit
    Spin Column Yeast Total Rna Purification Kit, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spin+column+yeast+total+rna+purification+kit/column+dna+extraction+gel+kit+sanprep/pmc12996981-50-6-13
    Average 86 stars, based on 1 article reviews
    spin column yeast total rna purification kit - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Isolation:

    Article Title: Production of mannitol from molasses and Jerusalem artichoke tubers at elevated temperatures using engineered Kluyveromyces marxianus through consolidated bioprocessing
    Article Snippet: .. After cultivating the strains in YPS (4% sucrose) at 42 °C for 24 h, the cells were recovered, and total RNA was isolated using the Spin Column Yeast Total RNA Purification Kit (Sangon Biotech, China). .. The genomic DNA in the sample was removed with gDNA Remover Mix. cDNA was synthesized with Hifair® V one-step RT-gDNA digestion SuperMix for qPCR (Yeasen, China).

    Article Title: Multiplexed Genome Editing and Transcriptional Knockdown in Yarrowia lipolytica by CRISPR-Cpf1 and an Orthogonal T7 System.
    Article Snippet: Yarrowia lipolytica, a nonconventional yeast, has become an industrial workhorse to synthesize valuable compounds, including lipids, oleochemicals, and nutraceuticals.. While the synthetic biology toolkits to engineer the genome and endogenous metabolic pathways are not as developed as Baker’s yeast, it has emerged as a promising microbial host for industrial applications.. In this study, we examined the multiplexed editing capability of the CRISPR-AsCpf1 coupled with gRNAs generated from either a yeast native promoter or an orthogonal T7 promoter, which yielded 73.3% editing efficiency for up to four target genes and 100% editing efficiency for two genes.

    Purification:

    Article Title: Production of mannitol from molasses and Jerusalem artichoke tubers at elevated temperatures using engineered Kluyveromyces marxianus through consolidated bioprocessing
    Article Snippet: .. After cultivating the strains in YPS (4% sucrose) at 42 °C for 24 h, the cells were recovered, and total RNA was isolated using the Spin Column Yeast Total RNA Purification Kit (Sangon Biotech, China). .. The genomic DNA in the sample was removed with gDNA Remover Mix. cDNA was synthesized with Hifair® V one-step RT-gDNA digestion SuperMix for qPCR (Yeasen, China).

    Article Title: Regulating the metabolic flux of pyruvate dehydrogenase bypass to enhance lipid production in Saccharomyces cerevisiae
    Article Snippet: .. A spin column yeast total RNA purification kit (Sangon Biotech, Shanghai, China) was used to extract total RNA according to the manufacturer’s instructions. cDNA synthesis was performed using fastKing gDNA dispelling RT supermix (TIANGEN Biotech [Beijing] Co., Ltd., KR118-02). .. Quantification of the cDNA copy number was accomplished using a SYBR Green PCR Kit (TIANGEN Biotech [Beijing] Co., Ltd., FP205-02) with a QuantStudio design and analysis system (QuantStudio 5 real-time fluorescence qPCR instrument).

    Article Title: Multiplexed Genome Editing and Transcriptional Knockdown in Yarrowia lipolytica by CRISPR-Cpf1 and an Orthogonal T7 System.
    Article Snippet: Yarrowia lipolytica, a nonconventional yeast, has become an industrial workhorse to synthesize valuable compounds, including lipids, oleochemicals, and nutraceuticals.. While the synthetic biology toolkits to engineer the genome and endogenous metabolic pathways are not as developed as Baker’s yeast, it has emerged as a promising microbial host for industrial applications.. In this study, we examined the multiplexed editing capability of the CRISPR-AsCpf1 coupled with gRNAs generated from either a yeast native promoter or an orthogonal T7 promoter, which yielded 73.3% editing efficiency for up to four target genes and 100% editing efficiency for two genes.

    Article Title: Identification of Flo11-like Adhesin in Schizosaccharomyces pombe and the Mechanism of Small-Molecule Compounds Mediating Biofilm Formation in Yeasts
    Article Snippet: .. The RNA was extracted from the biofilm samples in each Ep tube using the Spin Column Yeast Total RNA Purification Kit (Sangon Biotech, Shanghai, China), and cDNA synthesis was conducted using PrimeScriptTM RT Master Mix (TaKaRa, Kusatsu City, Japan). qPCR analyses of the C. albicans biofilm-related genes hwp1, ece1 , and als3 were performed using TB Green ® Premix Ex TaqTM II (TaKaRa, Japan), according to the manufacturer’s manual [ ], with the primers listed in , where the β-actin gene act1 was used as an internal control to standardize the transcripts of the genes. .. All analyses were conducted in the Applied Biosystems StepOnePlus Real-Time PCR System in triplicate for each sample.

    Article Title: Hypersecretory production of glucose oxidase in Pichia pastoris through combinatorial engineering of protein properties, synthesis, and secretion.
    Article Snippet: Funding information National Key Research and Development Program of China, Grant/Award Number: 2020YFA0908900 Abstract Glucose oxidase (EC 1.1.3.4, GOD) is a widely used industrial enzyme.. To construct a GOD‐hyperproducing Pichia pastoris strain, combinatorial strategies have been applied to improve GOD activity, synthesis, and secretion.. First, wild‐type GOD was subjected to saturation mutagenesis to obtain an improved variant, MGOD1 (V20W/T30S), with 1.7‐fold higher kcat/KM.

    Article Title: Valorization of xylose mother liquor into salidroside by engineered Kluyveromyces marxianus
    Article Snippet: .. Total RNA was extracted using the Spin Column Yeast Total RNA Purification Kit (Sangon Biotech, Shanghai), and first-strand cDNA was synthesized using ToloScript All-in-one RT EasyMix for qPCR (Tolo Biotech, Shanghai) following the manufacturers’ instructions. .. RT-qPCR was performed with 2 × Q3 SYBR qPCR Master Mix (Tolo Biotech, Shanghai) on a LightCycler 96 Real-Time PCR System (Roche, Switzerland) using gene-specific primers ( ).

    Article Title: Metabolic and transport engineering enable efficient de novo biosynthesis of patchoulol in Saccharomyces cerevisiae
    Article Snippet: .. Total RNA was extracted using the Spin Column Yeast Total RNA Purification Kit (Sangon Biotech, Shanghai, China) according to the manufacturer's instructions. ..

    cDNA Synthesis:

    Article Title: Regulating the metabolic flux of pyruvate dehydrogenase bypass to enhance lipid production in Saccharomyces cerevisiae
    Article Snippet: .. A spin column yeast total RNA purification kit (Sangon Biotech, Shanghai, China) was used to extract total RNA according to the manufacturer’s instructions. cDNA synthesis was performed using fastKing gDNA dispelling RT supermix (TIANGEN Biotech [Beijing] Co., Ltd., KR118-02). .. Quantification of the cDNA copy number was accomplished using a SYBR Green PCR Kit (TIANGEN Biotech [Beijing] Co., Ltd., FP205-02) with a QuantStudio design and analysis system (QuantStudio 5 real-time fluorescence qPCR instrument).

    Article Title: Identification of Flo11-like Adhesin in Schizosaccharomyces pombe and the Mechanism of Small-Molecule Compounds Mediating Biofilm Formation in Yeasts
    Article Snippet: .. The RNA was extracted from the biofilm samples in each Ep tube using the Spin Column Yeast Total RNA Purification Kit (Sangon Biotech, Shanghai, China), and cDNA synthesis was conducted using PrimeScriptTM RT Master Mix (TaKaRa, Kusatsu City, Japan). qPCR analyses of the C. albicans biofilm-related genes hwp1, ece1 , and als3 were performed using TB Green ® Premix Ex TaqTM II (TaKaRa, Japan), according to the manufacturer’s manual [ ], with the primers listed in , where the β-actin gene act1 was used as an internal control to standardize the transcripts of the genes. .. All analyses were conducted in the Applied Biosystems StepOnePlus Real-Time PCR System in triplicate for each sample.

    Real-time Polymerase Chain Reaction:

    Article Title: Identification of Flo11-like Adhesin in Schizosaccharomyces pombe and the Mechanism of Small-Molecule Compounds Mediating Biofilm Formation in Yeasts
    Article Snippet: .. The RNA was extracted from the biofilm samples in each Ep tube using the Spin Column Yeast Total RNA Purification Kit (Sangon Biotech, Shanghai, China), and cDNA synthesis was conducted using PrimeScriptTM RT Master Mix (TaKaRa, Kusatsu City, Japan). qPCR analyses of the C. albicans biofilm-related genes hwp1, ece1 , and als3 were performed using TB Green ® Premix Ex TaqTM II (TaKaRa, Japan), according to the manufacturer’s manual [ ], with the primers listed in , where the β-actin gene act1 was used as an internal control to standardize the transcripts of the genes. .. All analyses were conducted in the Applied Biosystems StepOnePlus Real-Time PCR System in triplicate for each sample.

    Article Title: Valorization of xylose mother liquor into salidroside by engineered Kluyveromyces marxianus
    Article Snippet: .. Total RNA was extracted using the Spin Column Yeast Total RNA Purification Kit (Sangon Biotech, Shanghai), and first-strand cDNA was synthesized using ToloScript All-in-one RT EasyMix for qPCR (Tolo Biotech, Shanghai) following the manufacturers’ instructions. .. RT-qPCR was performed with 2 × Q3 SYBR qPCR Master Mix (Tolo Biotech, Shanghai) on a LightCycler 96 Real-Time PCR System (Roche, Switzerland) using gene-specific primers ( ).

    Control:

    Article Title: Identification of Flo11-like Adhesin in Schizosaccharomyces pombe and the Mechanism of Small-Molecule Compounds Mediating Biofilm Formation in Yeasts
    Article Snippet: .. The RNA was extracted from the biofilm samples in each Ep tube using the Spin Column Yeast Total RNA Purification Kit (Sangon Biotech, Shanghai, China), and cDNA synthesis was conducted using PrimeScriptTM RT Master Mix (TaKaRa, Kusatsu City, Japan). qPCR analyses of the C. albicans biofilm-related genes hwp1, ece1 , and als3 were performed using TB Green ® Premix Ex TaqTM II (TaKaRa, Japan), according to the manufacturer’s manual [ ], with the primers listed in , where the β-actin gene act1 was used as an internal control to standardize the transcripts of the genes. .. All analyses were conducted in the Applied Biosystems StepOnePlus Real-Time PCR System in triplicate for each sample.

    Synthesized:

    Article Title: Valorization of xylose mother liquor into salidroside by engineered Kluyveromyces marxianus
    Article Snippet: .. Total RNA was extracted using the Spin Column Yeast Total RNA Purification Kit (Sangon Biotech, Shanghai), and first-strand cDNA was synthesized using ToloScript All-in-one RT EasyMix for qPCR (Tolo Biotech, Shanghai) following the manufacturers’ instructions. .. RT-qPCR was performed with 2 × Q3 SYBR qPCR Master Mix (Tolo Biotech, Shanghai) on a LightCycler 96 Real-Time PCR System (Roche, Switzerland) using gene-specific primers ( ).

    other:

    Article Title: Expression, optimization and biological activity analysis of recombinant type III collagen in Komagataella phaffii.
    Article Snippet: Type III collagen, a fundamental constituent of the extracellular matrix (ECM), is extensively utilized across the biomedicine, tissue engineering, and cosmetic industries because of its exceptional biocompatibility and biodegradability.. Despite its widespread application, traditional methods of collagen production are often hindered by the limit yield, potential immunogenicity, and batch-to-batch variability.. In the present study, we describe a synthetic biological approach for expressing full-length recombinant type III collagen (RCIII) in the Komagataella phaffii system.



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